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Rapid method for on-site determination of phenolic contaminants in water using a disposable biosensor

Yuanting LI, Dawei LI, Wei SONG, Meng LI, Jie ZOU, Yitao LONG

《环境科学与工程前沿（英文）》 2012年 第6卷 第6期   页码 831-838 doi: 10.1007/s11783-012-0393-z

摘要： A disposable biosensor was fabricated using single-walled carbon nanotubes, gold nanoparticles and tyrosinase (SWCNTs-AuNPs-Tyr) modified screen-printed electrodes. The prepared biosensor was applied to the rapid determination of phenolic contaminants within 15 minutes. The SWCNTs-AuNPs-Tyr bionanocomposite sensing layer was characterized with scanning electron microscopy, electrochemical impedance spectroscopy and cyclic voltammetry methods. The characterization results revealed that SWCNTs could lead to a high loading of tyrosinase (Tyr) with the large surface area and the porous morphology, while AuNPs could retain the bioactivity of Tyr and enhance the sensitivity. The detection conditions, including working potential, pH of supporting electrolyte and the amount of Tyr were optimumed. As an example, the biosensor for catechol determination displayed a linear range of 8.0 × 10 to 2.0 × 10 mol·L with a detection limit of 4.5 × 10 mol·L (S/ = 3). This method has a rapid response time within 10 s, and shows excellent repeatability and stability. Moreover, the resulting biosensor could be disposable, low-cost, reliable and easy to carry. This kind of new Tyr biosensor provides great potential for rapid, on-site and cost-effective analysis of phenolic contaminants in environmental water samples.

关键词： on-site determination     tyrosinase biosensor     phenolic contaminants     single-walled carbon nanotubes     gold nanoparticles     screen-printed electrodes    

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Performance evaluation on the pollution control against wet weather overflow based on on-site coagulation

Zongqun Chen, Wei Jin, Hailong Yin, Mengqi Han, Zuxin Xu

《环境科学与工程前沿（英文）》 2021年 第15卷 第6期 doi: 10.1007/s11783-021-1400-z

摘要： Abstract • A way for overflow control based on on-site coagulation/flocculation was proposed. • Coagulant and flocculant dose were optimized based on pollutant removal performance. • Settling time of 5 min is enough in a proper transmission distance. • Fast removal of particulate pollutants could be achieved under varied flow. The pollution caused by wet weather overflow in urban drainage systems is a main factor causing blackening an odorization of urban rivers. The conventional overflow treatment based on coagulation/flocculation in terminal drainage systems requires relatively large space and long retention time demand that makes it not applicable in crowded urban drainage systems or under heavy rains. On-site coagulation/flocculation in terminal drainage pipes was proposed in this study which was aimed to transfer the coagulation/flocculation process to the inside of pipes at the terminal drainage system to save space and reduce the retention time of the coagulation/flocculation process. The optimized dose of chemicals was studied first which was 80 mg/L of coagulant and 0.8 mg/L of flocculant. Settling for only 5 min can remove most of the pollutants at 406.5 m of transmission distance. In addition, the relation of wet weather overflow rate and concentration of pollution load on the on-site coagulation/flocculation process was investigated, which indicated that high removal of pollutant was gained at a large range of flow velocity and pollutant concentration. Finally, the study confirmed electric neutralization, bridging, and net capture as the major mechanisms in this process, and further optimization was proposed. The proposed process can reduce much turbidity, chemical oxygen demand, and total phosphorous, but hardly remove soluble ammonia and organics. This work provides scientific guidance to address wet weather overflow in terminal drainage pipes.

关键词： Wet weather overflow     On-site coagulation/flocculation     Fast removal of particulate pollutants    

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激光再制造技术与应用发展研究

姚喆赫,姚建华,向巧

《中国工程科学》 2020年 第22卷 第3期   页码 63-70 doi: 10.15302/J-SSCAE-2020.03.011

摘要：

激光再制造技术作为制造技术创新的前沿领域，深刻改变着高端装备的设计与运行方式，是绿色再制造的重要支撑技术，是促进制造产业可持续发展的重要力量。本文从激光再制造技术的宏观需求入手，梳理了激光再制造技术的发展及应用现状，凝练了激光再制造技术和产业在发展过程中面临的挑战，进而提出了面向2025 年、2035 年以及2050 年的分阶段发展目标。针对当前我国激光再制造技术在专用材料、核心器件、再制造理念与标准体系等方面存在的问题与挑战，研究提出：加强战略层面的积极引导；构建激光增材再制造材料基因组体系；完善标准化体系和高层次应用型人才培养体系；加大应用推广力度，引导行业整合；建立合作平台，加快关键共性技术创新发展，为激光再制造技术及其产业发展提供支撑。

关键词： 激光再制造，多能场，现场再制造，全生命周期    

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Influence of site conditions on seismic design parameters for foundations as determined via nonlinearsite response analysis

Muhammad Tariq A. CHAUDHARY

《结构与土木工程前沿（英文）》 2021年 第15卷 第1期   页码 275-303 doi: 10.1007/s11709-021-0685-0

摘要： Site conditions, including geotechnical properties and the geological setting, influence the near-surface response of strata subjected to seismic excitation. The geotechnical parameters required for the design of foundations include mass density ( ), damping ratio ( ), shear wave velocity ( ), and soil shear modulus ( ). The values of the last three parameters are sensitive to the level of nonlinear strain induced in the strata due to seismic ground motion. In this study, the effect of variations in soil properties, such as plasticity index ( ), effective stress ( ), over consolidation ratio (OCR), impedance contrast ratio ( ) between the bedrock and the overlying strata, and depth of soil strata over bedrock ( ), on seismic design parameters ( , , and ) was investigated for National Earthquake Hazards Reduction Program (NEHRP) site classes C and D, through 1D nonlinear seismic site response analysis. The Morris one-at-a-time (OAT) sensitivity analysis indicated that , , and were significantly influenced by variations in , while affected more than it affected and . However, the influence of on these parameters was less significant. It was also found that variations in soil properties influenced seismic design parameters in soil type D more significantly than in soil type C. Predictive relationships for , , and were derived based on the 1D seismic site response analysis and sensitivity analysis results. The , , and values obtained from the analysis were compared with the corresponding values in NEHRP to determine the similarities and differences between the two sets of values. The need to incorporate and in the metrics for determining , , and for the seismic design of foundations was highlighted.

关键词： site effects     1D seismic site response analysis     sensitivity analysis     foundations     shear wave velocity     soil shear modulus    

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Development and application of an off-site maintenance system in the petrochemical industry

Ruisheng YONG, Yanbing YE, Hanbin LUO, Lieyun DING

《工程管理前沿（英文）》 2017年 第4卷 第2期   页码 111-126 doi: 10.15302/J-FEM-2017027

摘要： On-site maintenance (ONSM) is critical to ensuring the safety of equipment in the petrochemical industry. However, many accidents occur during ONSM processes because of unstable equipment, complicated work environment, and human error. To reduce congestion and exposure to hazards during on-site operations and thereby reduce the probability of accidents, off-site maintenance (OFSM) is proposed based on Energy Release Theory (ERT). The information requirements for OFSM are analyzed. A support system for OFSM, which makes use of visualization, information management, and localization capabilities, is developed. A case study utilizing OFSM and its support system for an oil-loading facility of a petrochemical company is conducted. The application results indicated that implementation of OFSM system can significantly reduce the operation risks and can improve the operation efficiency.

关键词： on-site maintenance     petrochemical industry     safety     off-site maintenance system    

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Progress in studies of sex determination mechanisms and sex control techniques in

Qian ZHOU,Songlin CHEN

《农业科学与工程前沿（英文）》 2016年 第3卷 第2期   页码 113-123 doi: 10.15302/J-FASE-2016093

摘要： The (half-smooth tongue sole) is a marine flatfish of great commercial value for fisheries and aquaculture in China. It has a female heterogametic sex determination system (ZW/ZZ) and environmental factors can induce sex-reversal of females to phenotypic males, suggesting that it is a promising model for the study of sex determination mechanisms. Additionally, females grow much faster than males and it is feasible to improve the aquaculture production through sex control techniques. This paper reviews the progress in research on sex determination mechanisms research in our laboratory. We have completed whole-genome sequencing and revealed the genome organization and sex chromosome evolution of . A putative male determining gene was identified and DNA methylation was verified as having a crucial role in the sex reversal process. Genetic maps and sex-specific biomarkers have been used in a marker-assisted selection breeding program and for differentiation of the fish sex. Development and improvement of sex control technologies, including artificial gynogenesis and production of breeding fry with high proportion of females, is also reviewed. These research advances have provided insight into the regulation of sex determination and enabled efficient sex management in artificial culturing of .

关键词： Cynoglossus semilaevis     sex determination     sex control technique    

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An integrated approach to site-specific management zone delineation

Yuxin MIAO, David J. MULLA, Pierre C. ROBERT

《农业科学与工程前沿（英文）》 2018年 第5卷 第4期   页码 432-441 doi: 10.15302/J-FASE-2018230

摘要：

Dividing fields into a few relatively homogeneous management zones (MZ) is a practical and cost-effective approach to precision agriculture. There are three basic approaches to MZ delineation using soil and/or landscape properties, yield information, and both sources of information. The objective of this study is to propose an integrated approach to delineating site-specific MZ using relative elevation, organic matter, slope, electrical conductivity, yield spatial trend map, and yield temporal stability map (ROSE-YSTTS) and evaluate it against two other approaches using only soil and landscape information (ROSE) or clustering multiple year yield maps (CMYYM). The study was carried out on two no-till corn-soybean rotation fields in eastern Illinois, USA. Two years of nitrogen (N) rate experiments were conducted in Field B to evaluate the delineated MZs for site-specific N management. It was found that in general the ROSE approach was least effective in accounting for crop yield variability (8.0%–9.8%), while the CMYYM approach was least effective in accounting for soil and landscape (8.9%–38.1%), and soil nutrient and pH variability (9.4%–14.5%). The integrated ROSE-YSTTS approach was reasonably effective in accounting for the three sources of variability (38.6%–48.9%, 16.1%–17.3% and 13.2%–18.7% for soil and landscape, nutrient and pH, and yield variability, respectively), being either the best or second best approach. It was also found that the ROSE-YSTTS approach was effective in defining zones with high, medium and low economically optimum N rates. It is concluded that the integrated ROSE-YSTTS approach combining soil, landscape and yield spatial-temporal variability information can overcome the weaknesses of approaches using only soil, landscape or yield information, and is more robust for MZ delineation. It also has the potential for site-specific N management for improved economic returns. More studies are needed to further evaluate their appropriateness for precision N and crop management.

关键词： economically optimum nitrogen rate     fuzzy cluster analysis     precision nitrogen management     site-specific management     soil landscape property     yield map    

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中国核电工程场地设计地震动参数确定相关问题

李小军,贺秋梅,侯春林

《中国工程科学》 2013年 第15卷 第4期   页码 75-82

摘要：

基于中国核电工程选址和建设的实际工作，探讨了核电工程场地设计地震动参数确定几个环节中的关键问题，包括不同地震危险性分析方法的采用、地震动衰减关系的选取和设计地震动参数的综合取值等。分析了地震动衰减关系不确定性和弥散地震衰减关系等问题处理方式的变迁，比较分析了地震危险性分析确定性方法中构造地震、弥散地震的计算结果和概率方法计算结果的差异及对设计地震动参数确定的控制作用。研究表明：a.综合考虑地震危险性分析的确定性方法和概率方法的计算结果已成为中国核电工程场地地震安全性评价中确定设计地震动参数的基本思路，其中弥散地震是一个需要特殊考虑的问题；b.确定性方法和概率方法计算结果对场地设计地震动参数的控制作用受区域地震活动性强弱的影响；c.在地震活动性较弱的地区，确定性方法特别是弥散地震计算结果基本上控制着场地设计地震动参数的取值，而在地震活动性相对较强的地区，更倾向于由概率方法计算结果控制。

关键词： 核电厂     设计地震动     确定性方法     概率方法     地震动衰减关系     弥散地震     不确定性    

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Site-directed mutagenesis of long QT syndrome KCNQ1 gene

LI Wei, WANG Bin, XU Qiumei, KE Qinmei, YANG Junguo, DU Rong, TIAN Li, WANG Qing

《医学前沿（英文）》 2008年 第2卷 第1期   页码 100-104 doi: 10.1007/s11684-008-0018-x

摘要： To construct a polymerase chain reaction (PCR) site-directed mutagenesis of the long QT syndrome KCNQ1 gene , two sets of primers were designed according to the sequence of KCNQ1 cDNA and a mismatch was introduced into primers. Mutagenesis was performed in a two-step PCR. The amplified fragments from the third PCR which contained the mutation site were sub-cloned into the T-vector pCR2.1. Then, the fragments containing the mutation site was obtained from pCR2.1 using restriction enzymes digestion and inserted into the same restriction site of pIRES-EGFP-KCNQ1. The sequencing analysis shows that the mutation site was correct. Mutation from A to G in site 983 of KCNQ1 cDNA was found. Using the Effectene transfection reagent, pIRES-EGFP-KCNQ1 (G983A) was transfected into HEK cells successfully. These results may shed light on further functional study of KCNQ1 gene.

关键词： restriction     digestion     syndrome     sequence     site-directed mutagenesis    

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Winner determination problem with loss-averse buyers in reverse auctions

Xiaohu QIAN, Min HUANG, Yangyang YU, Xingwei WANG

《工程管理前沿（英文）》 2017年 第4卷 第2期   页码 212-220 doi: 10.15302/J-FEM-2017019

摘要： Reverse auctions have been widely adopted for purchasing goods and services. This paper considers a novel winner determination problem in a multiple-object reverse auction in which the buyer involves loss-averse behavior due to uncertain attributes. A corresponding winner determination model based on cumulative prospect theory is proposed. Due to the NP-hard characteristic, a loaded route strategy is proposed to ensure the feasibility of the model. Then, an improved ant colony algorithm that consists of a dynamic transition strategy and a Max-Min pheromone strategy is designed. Numerical experiments are conducted to illustrate the effectiveness of the proposed model and algorithm. We find that under the loaded route strategy, the improved ant colony algorithm performs better than the basic ant colony algorithm. In addition, the proposed model can effectively characterize the buyer’s loss-averse behavior.

关键词： reverse auction     loss aversion     winner determination     improved ant colony algorithm    

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Construction Technology of Off-Site Precast Concrete Buildings

Ting Gong,Jian Yang,Hao Hu,Feng Xu

《工程管理前沿（英文）》 2015年 第2卷 第2期   页码 122-124 doi: 10.15302/J-FEM-2015039

摘要： The industrialization of building construction is gradually becoming a new choice of construction technology driven by some great advantages such as high efficiency in construction, low energy consumption and environmental friendliness. Construction management plays an important part to ensure the quality of projects. A comparison between traditional on-site and modern off-site construction methods has been conducted. The streamlining of the process including the fabrication, transportation, stacking and assembling of precast components has also been analyzed with a discussion on the BIM application in the process.

关键词： off-site construction     precast concrete     modular construction     construction management     building information model (BIM)    

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Comments on “Determination of heterozygosity for avirulence/virulence loci through sexual hybridization

Ralf T. Voegele

《农业科学与工程前沿（英文）》 2017年 第4卷 第1期   页码 121-122 doi: 10.15302/J-FASE-2017142

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Evaluation of site response characteristic using nonlinear method (Case study: Babol, Iran)

Asskar Janalizadeh CHOOBBASTI, Sadegh REZAEI, Farzad FARROKHZAD, Pedram Haidarzaeh AZAR

《结构与土木工程前沿（英文）》 2014年 第8卷 第1期   页码 69-82 doi: 10.1007/s11709-014-0231-4

摘要： In this the present study, an attempt has been made to evaluate the seismic hazard considering local site effects by carrying out detailed geotechnical site characterization in Babol, Iran. Use of geotechnical data and synthesis of drilling data extracted from the Babol’s subsurface database have enabled authors to determine the geotechnical properties of each site. These data are consisted of twenty five boreholes up to depth of 40 m. Based on the obtained data from geotechnical investigation the study area is divided to five zones. Dynamic analysis was performed in time domain, using fully nonlinear model by PLAXIS. A series of analysis in the study area showed the site period, ranging from 0.4 to 0.8 s. Finally the obtained response spectra from fully nonlinear method were compared with site response spectra of Iran’s 2800 (earthquake) code.

关键词： fully nonlinear method     site effect     dynamic analysis     response spectra    

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Experimental determination of Cd

Farhah Amalya ISMAIL, Ahmad Zaharin ARIS

《环境科学与工程前沿（英文）》 2013年 第7卷 第3期   页码 356-364 doi: 10.1007/s11783-013-0488-1

摘要： Carbonate shells have an astonishing ability in the removal of Cd in a short time period with emphasis on being a low cost adsorbent. In the present study, the sorption capacity of carbonate shells was studied for Cd in batch experiments. The influence of different carbonate shell sizes and physico-chemical factors were evaluated and the results were analyzed for its correlation matrices by using Predictive Analytics Software (PASW). The mineralogy state of aqueous solution regarding the saturation index was simulated using PHREEQC to identify the Cd uptake mechanism. The Cd uptake rates were calculated as well as Ca , concentration, pH, ambient humidity and temperature were measured. Cd removal of 91.52% was achieved after 5 h adsorption. The adsorption efficiencies were significantly influenced by pH as they increased with the increase of pH from acidic solution (5.50±0.02) to slightly alkaline (7.60±0.05). In addition, the mineralogy state of aqueous solution calculated from PHREEQC confirmed that the increment of Ca and concentrations in solution was attributed to the dissolution of carbonate shells. Moreover, the ion exchange adsorption mechanism of Cd toward Ca was identified as the process involved in Cd uptake.

关键词： carbonate shell     cadmium     heavy metal     adsorption mechanism     saturation state    

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A simplified method for the determination of vertically loaded pile-soil interface parameters in layered

Jiu-jiang WU,Yan LI,Qian-gong CHENG,Hua WEN,Xin LIANG

《结构与土木工程前沿（英文）》 2016年 第10卷 第1期   页码 103-111 doi: 10.1007/s11709-015-0328-4

摘要： The numerical analysis of pile-soil interaction commonly requires a lot of trial works to determine the interface parameters and the accuracy cannot be ensured normally. Considering this, this paper first conducts a sensitivity analysis to figure out the influence of interface parameters on the bearing behavior of a single pile in sand. Then, a simplified method for the determination of pile-soil interface parameters in layered soil is proposed based on the parameter studies. Finally, a filed loading test is used for the validation of the simplified method, and the calculated results agree well with the monitoring data. In general, the simplified method proposed in this paper works with higher accuracy and consumes less time compared with the traditional trial works, especially on the determinations of interfacial cohesive and interfacial friction angle.

关键词： determination of interface parameters     pile-soil interaction     FLAC3D     sensitivity analysis     layered soil    

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标题 作者 时间 类型 操作

Rapid method for on-site determination of phenolic contaminants in water using a disposable biosensor

Yuanting LI, Dawei LI, Wei SONG, Meng LI, Jie ZOU, Yitao LONG

期刊论文

Performance evaluation on the pollution control against wet weather overflow based on on-site coagulation

Zongqun Chen, Wei Jin, Hailong Yin, Mengqi Han, Zuxin Xu

期刊论文

激光再制造技术与应用发展研究

姚喆赫,姚建华,向巧

期刊论文

Influence of site conditions on seismic design parameters for foundations as determined via nonlinearsite response analysis

Muhammad Tariq A. CHAUDHARY

期刊论文

Development and application of an off-site maintenance system in the petrochemical industry

Ruisheng YONG, Yanbing YE, Hanbin LUO, Lieyun DING

期刊论文

Progress in studies of sex determination mechanisms and sex control techniques in

Qian ZHOU,Songlin CHEN

期刊论文

An integrated approach to site-specific management zone delineation

Yuxin MIAO, David J. MULLA, Pierre C. ROBERT

期刊论文

中国核电工程场地设计地震动参数确定相关问题

李小军,贺秋梅,侯春林

期刊论文

Site-directed mutagenesis of long QT syndrome KCNQ1 gene

LI Wei, WANG Bin, XU Qiumei, KE Qinmei, YANG Junguo, DU Rong, TIAN Li, WANG Qing

期刊论文

Winner determination problem with loss-averse buyers in reverse auctions

Xiaohu QIAN, Min HUANG, Yangyang YU, Xingwei WANG

期刊论文

Construction Technology of Off-Site Precast Concrete Buildings

Ting Gong,Jian Yang,Hao Hu,Feng Xu

期刊论文

Comments on “Determination of heterozygosity for avirulence/virulence loci through sexual hybridization

Ralf T. Voegele

期刊论文

Evaluation of site response characteristic using nonlinear method (Case study: Babol, Iran)

Asskar Janalizadeh CHOOBBASTI, Sadegh REZAEI, Farzad FARROKHZAD, Pedram Haidarzaeh AZAR

期刊论文

Experimental determination of Cd

Farhah Amalya ISMAIL, Ahmad Zaharin ARIS

期刊论文

A simplified method for the determination of vertically loaded pile-soil interface parameters in layered

Jiu-jiang WU,Yan LI,Qian-gong CHENG,Hua WEN,Xin LIANG

期刊论文